The researchers enhanced the resistant starch content of high amylose rice starch by using heat-moisture treatment. By optimizing the treatment conditions, they increased the resistant starch content from 32.1% to 46.4%. The treatment changed the starch granules' structure, reduced solubility and swelling power, and altered the X-ray diffraction pattern. Gelatinization temperature increased, while gelatinization enthalpy decreased. This method can be used to produce high-yield resistant starch from high amylose rice starch for industrial applications.