Phages can break down the structure of E. faecalis biofilms by killing bacterial cells and affecting extracellular substances. Two types of phages were tested on 1-day and 7-day-old biofilms. Phages 2e mainly stopped planktonic cell growth but didn't impact the biofilm much. Phages 3e were effective at dispersing biofilms and reducing extracellular substances. When used together, the phage cocktail had a strong antimicrobial effect on both planktonic and biofilm bacteria, reducing polysaccharides, proteins, and eDNA levels in 1-day-old biofilms.