Stem cell cryopreservation aims to protect cells during freezing and thawing to maintain their ability to function normally. Cryoinjuries can occur due to cell dehydration or ice formation inside cells. Using the right freezing method and cryoprotective agents like glycerol and DMSO can improve cell recovery after thawing. While current freezing techniques are common, there are still unanswered questions about the best ways to preserve living cells.