Coomassie Blue Dye in Bradford Assay interacts with DNA, impacting accuracy.
The Bradford Assay is a method to measure protein levels in a sample using Coomassie Blue dye. This dye binds to proteins, allowing them to be detected by a spectrophotometer. However, when DNA is present in the sample, it can interfere with the accuracy of the protein measurement. Researchers found that Coomassie Blue dye interacts with DNA from salmon testes at a much slower rate compared to its interactions with standard proteins like bovine serum albumin. This interaction could affect the accuracy of protein measurements in samples containing both DNA and proteins.