Silymarin and PKA inhibitor modulate mouse sperm motility, suggesting glycolysis-PKA pathway regulates fertility.
The scientists wanted to see if silymarin and a protein inhibitor could change how glucose affects mouse sperm movement. They put the sperm in fluids with high or normal glucose levels and used tools to watch how they moved. Sperm in high glucose moved more at 120 minutes. Silymarin didn't do much in normal glucose, but it slowed down the overactive sperm in high glucose after 120 minutes. The protein inhibitor also slowed sperm movement, especially in high glucose with or without silymarin. This shows that the protein inhibitor affects how glucose boosts sperm movement. Silymarin helps maintain normal sperm movement but stops glucose from making them move too fast.