The study looked at how chitosan in the diet affects lead levels in the blood and tissues of rats exposed to lead. Rats were given lead and different levels of chitosan in their diet for 8 weeks. The results showed that chitosan reduced lead levels in the blood and tissues, with a significant decrease in blood lead levels. Chitosan also had an impact on the levels of iron, zinc, and calcium in the blood and tissues, with varying effects depending on the chitosan concentration. Overall, the study suggests that chitosan can help reduce lead absorption and may affect the levels of essential minerals in the body.