Lotus species like Lotus corniculatus are important forage crops due to their ability to grow in various soil conditions, withstand heavy grazing, and not cause bloat. However, specific challenges like seed shattering and genetic limitations hinder their development. Scientists have successfully transformed several Lotus species using Agrobacterium, a gene transfer system. This genetic engineering approach can help introduce beneficial traits into Lotus plants more quickly and efficiently. Different Lotus species have varying regeneration efficiencies, but overall, they can be easily maintained in the lab for transformation experiments. The protocols outlined in the study provide guidance for researchers interested in adapting these methods for transforming their preferred Lotus species.